Anti -FKBP4 (FK506 Binding Protein 4, 59kD, FK506 binding protein 4, FKBP52, FKBP59, HBI,
Hsp56, PPIase, p52, HSP binding immunophilin, T-cell FK506-binding protein, peptidylprolyl
cis-trans isomerase, rotamase)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH |
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| Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with | | | a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR co-chaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A- binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90- binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5). | | | | | | Positive Control: | | | Whole tissue extracts from rat kidney and rat and mouse testes. | | | Catalog # | F4150-16B1 | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot:1:2000 | | Immunohistochemistry:1:250 | | Immunoprecipitation:1:250 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 10F700 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS in 0.09% sodium azide and 50% glycerol. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic peptide corresponding to the residues of human FKBP54. | | Specificity | Recognizes human FKBP54. Species Crossreactivity: canine, hamster, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -FKBP4 (FK506 Binding Protein 4, 59kD, FK506 binding protein 4, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52, HSP binding immunophilin, T-cell FK506-binding protein, peptidylprolyl cis-trans isomerase, rotamase) |
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