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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)

Anti -Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase
fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of two extended beta-sheets flanked by four alpha-helices. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34,000kD protein, which has been subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.
Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Catalog #F4193-02
Recommended DilutionImmunofluorescence: 1:500-1:2500 for yeast fibrillarin; 1:250 for mammalian fibrillarin.
Western Blot: 1:1000 (cell lysates)-1:5000 (nuclear fractions) for yeast fibrillarin; 1:250 for mammalian fibrillarin. ECL detection was used.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No8A152
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenYeast fibrillarin.
SpecificityRecognizes yeast fibrillarin. Species crossreactivity: human, rat, plant, Drosophila, C. elegans and S. pombe.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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