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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome.
Catalog #	F4195
Applications	Suitable for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution	Immunohistochemistry: Acetone-fixed frozen sections
	Western Blot: 1-2ug/ml for 2hrs at RT.
	Optimal dilutions to be determined by the researcher.
Positive Control	Normal skin fibroblasts
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	0.N.294
Host	Mouse
Source	Bovine
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide, see F4195X.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Microfibrils from zonular apparatus of bovine eye. Epitope
Specificity	Recognizes bovine Fibrillin-1, 350kD. In Immunohistochemistry staining of human skin, decorates elastic framework, more specifically the oxytalantic network close to the epidermis. Cultures of normal human skin fibroblasts show discrete staining of extracellular fibrils. Does not recognize Fibrillin 2.
Species Crossreactivity	Human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.