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You are here:Home » Antibodies » Abs to Fibrillin » Anti -Fibrillin 1

Anti -Fibrillin 1

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome.
Catalog #F4195
ApplicationsSuitable for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry: Acetone-fixed frozen sections
Western Blot: 1-2ug/ml for 2hrs at RT.
Optimal dilutions to be determined by the researcher.
Positive ControlNormal skin fibroblasts
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.294
HostMouse
SourceBovine
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide, see F4195X.
PurityPurified by Protein G affinity chromatography.
ImmunogenMicrofibrils from zonular apparatus of bovine eye. Epitope
SpecificityRecognizes bovine Fibrillin-1, 350kD. In Immunohistochemistry staining of human skin, decorates elastic framework, more specifically the oxytalantic network close to the epidermis. Cultures of normal human skin fibroblasts show discrete staining of extracellular fibrils. Does not recognize Fibrillin 2.
Species CrossreactivityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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