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You are here:Home » Antibodies » Abs to Fibrillin » Anti -Fibrillin 1, exons 64-65

Anti -Fibrillin 1, exons 64-65

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IH IC
Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome.
Catalog #F4195-05
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:50-1:100
ELISA: 1:1000-1:2000
Western Blot: 1:200-1:500
Immunoprecipitation: 1:100-1:200
Optimal dilutions to be determined by the researcher.
NoteIn many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris, pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 40% glycerol. No preservative added.
PuritySerum
ImmunogenHis-tagged fusion protein containing exons 64-65 of human fibrillin-1
SpecificityReacts strongly with human and bovine fibrillin-1. Crossreactivity with fibrillin-2 may occur, but has not been determined.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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