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You are here:Home » Antibodies » Abs to Fibrillin » Anti -Fibrillin 2, exon 10, Gly-rich Domain

Anti -Fibrillin 2, exon 10, Gly-rich Domain

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IH IC
Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome.
Catalog #F4195-10
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:200-1:500
Immunohistochemistry/Immunocytochemistry: 1:50-1:100
ELISA: 1:1000-1:2000
Immunoprecipitation: 1:100-1:200
Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS.
PuritySerum
ImmunogenProtein fragment containing glycine-rich domain of exon 10 of human Fibrillin 2.
SpecificityRecognizes human Fibrillin 2. Does not crossreact with Fibrillin 1. Species Crossreactivity: bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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