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You are here:Home » Antibodies » Abs to Fibrillin » Anti -Fibrillin 1 (BSA & Azide Free)

Anti -Fibrillin 1 (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome.
Catalog #F4195X
ApplicationsSuitable for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry: Acetone-fixed and frozen sections
Western Blot: 1-2ug/ml for 2hrs RT
Optimal dilutions to be determined by the researcher.
HybridomaProduced by the fusion between BALB/c splenocytes and mouse myeloma P3 X 63A8.653 (NS1) cells.
Positive ControlNormal skin fibroblasts
Cellular LocalizationExtracellular microfibrils
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.294
HostMouse
SourceBovine
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See F4195.
PurityPurified by Protein G affinity chromatography.
ImmunogenMicrofibrils from zonular apparatus of bovine eye.
SpecificityRecognizes Fibrillin-1 (350kD). Reactivity with deletion mutants indicates that the epitope spans between aa451-909 of fibrillin-1. In Immunohistochemistry staining of human skin, decorates elastic framework. More specifically the oxytalantic network close to the epidermis. Cultures of normal human skin fibroblasts show discrete staining of extracellular fibrils. Species Crossreactivity: Bovine, human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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