| Human D-dimer (DD), a specific degradation product of cross-linked fibrin, is released into the bloodstream during clot breakdown. It is used as an indicator of a blood clots, venous thromboses or pulmonary embolisms. |
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| Catalog # | F4197-15 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications have not been tested. |
| Recommended Dilution | Sandwich ELISA: Plasma samples should be diluted with 10mM Tris-HCl buffer, pH 7.5, 1M sodium chloride, 0.1% Tween 20. To avoid non-specific binding, the final sodium chloride concentration in plasma samples should be 0.5M. |
| Western Blot: Non-reducing |
| Optimal dilutions to be determined by the researcher. |
| Recommended Pair | Suitable for use as the capture or solid phase antibody. |
| Matched to F4197-17C or F4197-17D as the detection or conjugate (label) antibody in a 1-step sandwich immunoassay. |
| Matched to F4197-17, F4197-18 and F4197-20 as the detection or conjugate (label) antibody in a 2-step |
| sandwich immunoassay. |
| Hydridoma | Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 1.B.669 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by Protein A sepharose chromatography. |
| Immunogen | Homogenized fibrin clot. |
| Specificity | Recognizes human D-dimer (DD), a specific degradation product of cross-linked fibrin, as well as a high molecular weight fibrin degradation products. Does not crossreact with D-monomer and fibrinogen. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
