| Fibrinogen is synthesized in the liver and platelets and plays a major role in the coagulation process. It consists of 6 polypeptide chains, two each of A alpha-, B beta- and gamma-chains and is a dimeric molecule, each subunit consisting of one of each chain type. Thrombin cleaves N-terminal peptides from each of the A alpha-and B beta-chains to form fibrin monomer. The newly exposed N-terminal sites spontaneously link to C-terminal sites of another fibrin molecule to form a fibrin chain. These are then crosslinked by activated Factor XIII which stabilizes the fibrin clot. Normal plasma levels of fibrinogen are around 2.6g/L. |
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| Catalog # | F4199-90B |
| Labeling Ratio | Fluorescein:antibody molar ratio: 2.8-3.3 |
| Absorbance ratio (A493/A276): 0.78±0.05 |
| Applications | Suitable for use in Immunofluorescence (direct or indirect) and Fluorescence microscopy. Other applications not tested. |
| Recommended Dilution | 1. Incident Light Excitation |
| Direct Immunofluorescence: 1:10-1:100 |
| Indirect Immunofluorescence: 1:20-1:400 |
| 2. Transmitted Light Excitation Direct Immunofluorescence | 1:5-1:40 |
| Indirect Immunofluorescence: 1:10-1:80 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Sheep |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 20mM Tris, 70mM sodium chloride, pH 8.0, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC). |
| Purity | Purified by affinity chromatography. |
| Immunogen | Purified fibrinogen derived from pooled human plasma. |
| Specificity | Prior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
