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You are here:Home » Antibodies » Abs to Fibrinogen » Anti -Fibrinogen (Coagulation Factor I) (FITC)

Anti -Fibrinogen (Coagulation Factor I) (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified IF Gel
Fibrinogen is synthesized in the liver and platelets and plays a major role in the coagulation process. It consists of 6 polypeptide chains, two each of A alpha-, B beta- and gamma-chains and is a dimeric molecule, each subunit consisting of one of each chain type. Thrombin cleaves N-terminal peptides from each of the A alpha-and B beta-chains to form fibrin monomer. The newly exposed N-terminal sites spontaneously link to C-terminal sites of another fibrin molecule to form a fibrin chain. These are then crosslinked by activated Factor XIII which stabilizes the fibrin clot. Normal plasma levels of fibrinogen are around 2.6g/L.
Catalog #F4199-90B
Labeling RatioFluorescein:antibody molar ratio: 2.8-3.3
Absorbance ratio (A493/A276): 0.78±0.05
ApplicationsSuitable for use in Immunofluorescence (direct or indirect) and Fluorescence microscopy. Other applications not tested.
Recommended Dilution1. Incident Light Excitation
Direct Immunofluorescence: 1:10-1:100
Indirect Immunofluorescence: 1:20-1:400
2. Transmitted Light Excitation Direct Immunofluorescence 1:5-1:40
Indirect Immunofluorescence: 1:10-1:80
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 20mM Tris, 70mM sodium chloride, pH 8.0, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by affinity chromatography.
ImmunogenPurified fibrinogen derived from pooled human plasma.
SpecificityPrior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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