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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B RIA

Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver. The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulfide-bound polypeptide chains named Aalpha, Bbeta and gamma. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains.
Catalog #	F4199-91
Applications	Suitable for use in ELISA, Western Blot and RIA. Other applications have not been tested.
Recommended Dilutions	ELISA: Binds human fibrinogen in solid-phase format.
	Western Blot (Non-reduced): Binds A-alpha chain of fibrinogen.
	RIA: Binds 125I-labeled fibrinogen.
	Optimal dilutions to be determined by the researcher.
Hybridoma	NS-1 myeloma cells with spleen cells from Balb/c mice.
Source	Culture
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	1.B.697
Host	Mouse
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.4, 0.01% sodium azide. Reconstitute with 500ul sterile ddH2O.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Human fibrinogen
Specificity	Recognizes human fibrinogen.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.