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You are here:Home » Antibodies » Abs to Fibrinogen » Anti -Fibrinogen (Coagulation Factor I)

Anti -Fibrinogen (Coagulation Factor I)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Fibrinogen, the precursor of fibrin, is the coagulable protein in the blood plasma of vertebrates. Fibrinogen consists as a dimer of 3 pairs of non-identical chains, alpha (Mr= 66,800), beta (Mr= 52,000) and gamma (Mr= 46,500) cross-linked by disulfide bonds in their N-terminal segments. The molecule has 2 terminal D domains and one central E domain, all three domains are separated when fibrinogen is degradated by plasmin. MW is 340kD (1). Concentration in human plasma is 2-3mg/ml (2).
Catalog #F4199-91B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000
Western Blot: Reacts with fibrinogen in non-reduced form only.
Optimal dilutions to be determined by the researcher.
Culture MediumRMPI 1640 with 2-10% fetal calf serum.
HybridomaX63-Ag8.653. Female CF1 x Balb/c mice
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F99
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
No stabilizing proteins added.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenFibrinogen isolated from human plasma.
SpecificityRecognizes human Fibrinogen. Reacts strongly with fibrinogen. Strong reaction is seen in ELISA with factor VIII HP directly coated onto the microtiterwell, also when tested in sandwich ELISA in combination with a polyclonal antibody against fibrinogen.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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