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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Fibrinogen, the precursor of fibrin, is the coagulable protein in the blood plasma of vertebrates. Fibrinogen consists as a dimer of 3 pairs of non-identical chains Aa (Mr= 66,800), Bb (Mr= 52,000) and g (Mr=46,500) cross-linked by disulfide bonds in their N-terminal segments. The molecule has 2 terminal D domains and one central E domain, all three domains are separated when fibrinogen is degradated by plasmin. MW is 340kD (1). Concentration in human plasma is 2-3mg/ml (2).
Catalog #	F4199-91C
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:8000
	Western Blot: 1:1000
	Optimal dilutions to be determined by the researcher.
Hybridoma	X63-Ag8.653. Female CF1 x Balb/c mice immunized by intraperitoneal injection.
Culture Medium	RPMI 1640 with 10% fetal calf serum
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F100
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Fibrinogen isolated from human plasma.
Specificity	Recognizes human fibrinogen degradation product D-fragment. Reacts with both purified D-monomer, fibrinogen and factor VIII. Epitope specificity differs from that of clone 6F99 as determined by inhibition ELISA. Reacts strongly with D-monomer. Strong reaction is seen in ELISA with antigen directly coated onto the microtiter plate or in sandwich ELISA in combination with a polyclonal antibody against fibrinogen. In Western Blot after SDS-PAGE, this antibody reacts with fibrinogen degradation product D-monomer in nonreduced forms to create a band of approximately 92kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.