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You are here:Home » Antibodies » Abs to Fibrinogen » Anti -Fibrinogen (Coagulation Factor I) (FITC)

Anti -Fibrinogen (Coagulation Factor I) (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E
Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking.
Catalog #F4199-91L
ApplicationsSuitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.
Recommended DilutionELISA: 1:20-1:80
Optimal dilutions to be determined by the researcher.
Excitation 492nm
Emission 515nm
Fluorescein/IgG protein Molar Ratio (F/P) ~1.5
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
PurityPurified by DEAE chromatography.
ImmunogenFibrinogen isolated from fresh plasma after removing prothrombin.
SpecificityRecognizes human fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Species Crossreactivity: Bovine, canine, feline, monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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