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You are here:Home » Antibodies » Abs to Fibrinogen » Anti -Fibrinogen (Coagulation Factor I) (FITC)

Anti -Fibrinogen (Coagulation Factor I) (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified
Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the
precursor of fibrin, which is the key protein constituting the network of the blood clot.
Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize
to fibrin which is stabilized by cross-linking.
Catalog #F4199-92J
ApplicationsSuitable for use in FLISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.
Recommended DilutionFLISA: 1:10-1:40
Immunofluorescence: 1:10-1:40
Optimal dilutions to be determined by the researcher.
Fluorescein/IgG protein molar ratio (F/P) ~2.2
Excitation 492nm
Emission 515nm
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
PurityPurified by DEAE chromatography.
ImmunogenFibrinogen isolated from fresh rat plasma after removing prothrombin
SpecificityRecognizes rat Fibrinogen. In immunoelectrophoresis and radial immunodiffusion against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Does not crossreact with any other component of rat plasma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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