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You are here:Home » Antibodies » Abs to Fibrinogen » Anti -Fibrinogen (Coagulation Factor I)

Anti -Fibrinogen (Coagulation Factor I)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum
Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the
precursor of fibrin, which is the key protein constituting the network of the blood clot.
Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers
polymerize to fibrin which is stabilized by cross-linking.
Catalog #F4199-92L
ApplicationsSuitable for use in IEP and RID. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceCanine
ConcentrationNot determined
FormSupplied as a lyophilized powder. No prevervatives added. Reconstitute with 1ml streil ddH2O. Dilute further with PBS.
PuritySerum
ImmunogenFibrinogen is isolated from fresh plasma after removing prothrombin.
SpecificityRecognizes canine Fibrogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal dog plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. The antiserum does not cross react with any other component of dog plasma. Inter- species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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