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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E

Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver. The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulfide-bound polypeptide chains named A-alpha, B-beta and gamma. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains. Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, A-alpha1-16) followed by Fibrinopeptide B (FPB, B-beta1-14) The resulting fibrin monomers polymerize in a half-overlap to form insoluble fibrin fibrils. The terms alpha, beta and gamma are used to indicate the removal of FPA and FPB. Polymerized fibrin is stabilized by the transglutaminase activated Factor XIII that forms amide linkages between gamma chains and, to a lesser extent, alpha chains of the fibrin molecules. Proteolysis of fibrinogen releases C-terminal residues from the A-alpha chain to produce fragment X (intact DE-D, which still clots). Fragment X is further degraded to non-clotting fragments Y (D-E) and D. Fragment Y can be digested into its components: D and E fragments. Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E. Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the gamma chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD. For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184kD for fragment DD, 92kD for D, 50kD for E, 1.54kD for FPA and 1.57kD for FPB (1-3).
Catalog #	F4200-15
Application	Suitable for use ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Human
Concentration	~2mg/ml
Form	Supplied as a liquid in 0.01M HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Fibrinogen purified from human plasma.
Specificity	Recognizes human Fibrinogen. Monospecific when analyzed by immunoelectrophoresis and ELISA. Weakly recognizes mouse, rat, rabbit, canine and porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.