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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -Fibrinopeptide A (FPA)

Anti -Fibrinopeptide A (FPA)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E Gel
Fibrinogen is an abundant plasma protein (5-10uM) produced in the
liver. The intact protein has a molecular weight of 340kD and is
composed of 3 pairs of disulfide-bound polypeptide chains named
Aalpha, Bbeta and gamma. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains. Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, Aalpha1-16) followed by Fibrinopeptide B (FPB, Bbeta1-14) and the fibrin monomers that result polymerize in a half-overlap fashion to form insoluble fibrin fibrils. The chains of fibrin are referred to as alpha, beta and gamma, due to the removal of FPA and FPB. The polymerised fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between gamma chains and to a lesser extent, alpha chains of the fibrin molecules. Proteolysis of fibrinogen by plasmin initially liberates C- terminal residues from the Aalpha chain to produce fragment X (intact D- E-D, which is still clottable). Fragment X is further degraded to non- clottable fragments Y (D-E) and D. Fragment Y can be digested into its constituent D and E fragments. Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E. Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the gamma chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD. For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184kD for fragment DD, 92kD for D, 50kD for E, 1.54kD for FPA and 1.57kD for FPB. This antibody is specific for fibrinopeptide A as demonstrated by immunoelectrophoresis and ELISA.
Applications:
Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Catalog #F4206-01
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~2mg/ml
FormSupplied in 10mM HEPES, 0.15 sodium chloride, pH7.4, 50% glycerol. No preservatives have been added.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic fibrinopeptide A alpha 1-16 conjugated to carrier.
SpecificityRecognizes human fibrinopeptide A (FPA).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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