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You are here:Home » Molecular Biology » MB-Carbohydrates, Glycoproteins » Fibroblast Activation Protein, Recombinant, Human (FAP)

Fibroblast Activation Protein, Recombinant, Human (FAP)

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Specifications

Human FAP (GenBank Accession No. NM_004460), (aa29-end) with C-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system.
Catalog #F4208-50H
Fibroblast Activation Protein (FAP) is a cell surface glycoprotein of reactive human fibroblasts. Among the normal adult organs tested, F4208-50H does not show immunoreactivity in immunohistological studies with either resting fibroblasts (fibrocytes) of the normal origin mesenchyme or the parenchymal cells. The single exception noted is a subset of endocrine cells in the pancreatic islets, presumably glucagon-producing A cells.
Specific Activity~10pmol/min/ug
Assay Buffer50mM Tris pH 7.5, 10mM MgCl2, 1mM MnCl2, 20uM substrate (A-P-AMC)
Assay conditionsIncubate 10 minutes at RT.
ApplicationsSuitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
Molecular Weight85.5kD
SourceSf9 insect cells
Purity~70%
Concentration~0.2mg/ml
FormSupplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM imidazole, 3mM DTT, 20% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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