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You are here:Home » Molecular Biology » Cells-Extracts, Lysates » Fibroblast Cell, 3T3/A31

Fibroblast Cell, 3T3/A31

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Specifications

Cellular protein preparation from murine 3T3 fibroblasts.
Catalog #F4209
ApplicationsSuitable for use in Western Blot as a Positive Control. Other applications not tested.
Recommended UsageImmunoblot: Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Immunoblot analysis.
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceMurine cells
Concentration~1mg/ml
FormSupplied as a liquid in modified RIPA buffer with non-reducing sample buffer. Cellular Protein Preparation: Cells were lysed in modified RIPA buffer (50mM Tris HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM sodium vanadate, 1mM sodium fluoride). Diluted with non-reducing sample buffer (31mM Tris HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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