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Specifications

Cellular protein preparation from murine 3T3 fibroblasts.
Catalog #	F4209
Applications	Suitable for use in Western Blot as a Positive Control. Other applications not tested.
Recommended Usage	Immunoblot: Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Immunoblot analysis.
	Optimal dilution to be determined by researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source	Murine cells
Concentration	~1mg/ml
Form	Supplied as a liquid in modified RIPA buffer with non-reducing sample buffer. Cellular Protein Preparation: Cells were lysed in modified RIPA buffer (50mM Tris HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM sodium vanadate, 1mM sodium fluoride). Diluted with non-reducing sample buffer (31mM Tris HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.