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You are here:Home » Biologicals-Cells, Plasma, Serum, Tissues » Cells- » Fibroblast Cell, 3T3, PDGF+/PDGF- Control Cells

Fibroblast Cell, 3T3, PDGF+/PDGF- Control Cells

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Specifications

This product consists of a pair of control extracts made from NIH3T3 cells. The cells are stimulated (+PDGF), or not (control) with Platelet Derived Growth Factor. Cells were extracted with modified RIPA buffer (50mM Tris, 150mM NaCl, 1% Triton-X 100, 0.5% deoxycholate, pH 7.3). The extracts can be used as controls for testing antibodies.
Catalog #F4209-01
ApplicationsSuitable for use in Western Blot (with phosphorylation site specific antibodies (PSSAs) to ERK1&2 [pTpY185/187] and PLCg-1 [pY783]). Other applications not tested.
Western Blot Conditions Prior to loading, heat the extract sample for 90 seconds at 95-100°C, then spin in a microfuge for 5 seconds at RT. We recommend using 20ml of the extract per lane for SDS-PAGE, and transferring onto a nitrocellulose membrane. At 20ml per lane, the amount provided is sufficient for four lanes on a standard mini-gel. The optimal amount of extract used should be determined empirically for each specific primary antibody tested.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityLysate
ConcentrationNot determined
FormStorage/Sample Buffer
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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