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You are here:Home » Molecular Biology » Growth Factors-FGF » Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)

Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)

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Specifications

Fibroblast Growth Factor-acidic (FGF-a or FGF-1) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. Human FGF-acidic is a 15.8kD protein containing 140 amino acid residues
Catalog #F4210-10
Biological ActivityHuman FGF-a is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors if  10ng/ml, corresponding to a specific activity of 1x10e5 units/mg.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile buffer and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight15.8kD
SourceE. coli
Purity95% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute in 5mM sodium phosphate, pH 7.0-7.2, to a concentration of < 0.5mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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