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Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)
Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)
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| Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. | | | Catalog # | F4210-10A | | Recombinant Human Fibroblast Growth Factor-acidic (FGF-1) is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15,967 Daltons. | | Amino Acid Sequence | MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D | | Biological Activity | The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <1ng/ml. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 15.967kD | | Source | Recombinant, Human expressed in E. coli | | Purity | 97% as determined by: RP-HPLC and SDS-PAGE. Chromatographically purified. | | Form | Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O to a concentration of 0.1-0.25mg/ml. Allow sample to sit for 5 minutes at 4ºC, spin to remove precipitant. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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