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You are here:Home » Molecular Biology » Growth Factors-FGF » Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)

Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)

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Specifications

Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Catalog #F4210-10A
Recombinant Human Fibroblast Growth Factor-acidic (FGF-1) is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15,967 Daltons.
Amino Acid SequenceMFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Biological ActivityThe ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <1ng/ml.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight15.967kD
SourceRecombinant, Human expressed in E. coli
Purity97% as determined by: RP-HPLC and SDS-PAGE. Chromatographically purified.
FormSupplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O to a concentration of 0.1-0.25mg/ml. Allow sample to sit for 5 minutes at 4ºC, spin to remove precipitant.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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