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You are here:Home » Molecular Biology » Growth Factors-FGF » Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)

Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)

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Specifications

Recombinant Human FGF-acidic (FGF-1) is a single, non-glycosylated, polypeptide chain containing 15aa and having a molecular weight of 17.4kD. The sequence of the first five N-terminal amino acids was determined to be Met-Phe-Asn-Leu-Pro. Contains less than 1% dimers and aggregates.
Catalog #F4210-10F
Source Recombinant Human FGF-acidic, expressed in E. coli.
Molecular weight 17.4kD
Endotoxin 0.1ng/ug (IEU/ug).
Activity
The ED50 as determined by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10ng/ml, corresponding to a specific activity of 1x10e5Units/mg.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Stable for at least 6 months at -20°C. Reconstitute with sterile deionized water to a concentration 100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.4kD
SourceE. coli
Purity95% (HPLC, FPLC, SDS-PAGE).
FormSupplied as a lyophilized powder from 10mM Tris, pH 7.6, 100mM sodium chloride. No preservative added. Reconstitute with sterile deionized water to a concentration 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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