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You are here:Home » Growth Factors, Cytokines » Growth Factors-FGF » Fibroblast Growth Factor, Acidic, Recombinant, Human (Acidic Fibroblast Growth Factor, aFGF, FGF-alpha, FGFA, Beta-endothelial Cell Growth Factor, ECGF-beta, ECGFB, ECGF, ECGFA, FGF1, GLIO703, Heparin-binding Growth Factor 1, HBGF1, HBGF-1)

Fibroblast Growth Factor, Acidic, Recombinant, Human (Acidic Fibroblast Growth Factor,
aFGF, FGF-alpha, FGFA, Beta-endothelial Cell Growth Factor, ECGF-beta, ECGFB, ECGF, ECGFA,
FGF1, GLIO703, Heparin-binding Growth Factor 1, HBGF1, HBGF-1)

Pricing

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Specifications

Catalog #F4210-10J
SourceRecombinant corresponding to 155aa of human Fibroblast Growth Factor-acidic expressed in E.coli is a single, non-glycosylated,polypeptide chain.
Molecular Weight~17.5kD
Biological ActivityThe ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is less then 10ng/ml, corresponding to a specific activity of 10Units/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of FGF-acidic as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.5kD
Purity~95% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesFibroblast Growth Factor 1 (Acidic)


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