Fibroblast Growth Factor, Acidic, Recombinant, Human (Acidic Fibroblast Growth Factor, aFGF, FGF-alpha, FGFA, Beta-endothelial Cell Growth Factor, ECGF-beta, ECGFB, ECGF, ECGFA, FGF1, GLIO703, Heparin-binding Growth Factor 1, HBGF1, HBGF-1)
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| Catalog # | F4210-10J | | Source | Recombinant corresponding to 155aa of human Fibroblast Growth Factor-acidic expressed in E.coli is a single, non-glycosylated,polypeptide chain. | | Molecular Weight | ~17.5kD | | Biological Activity | The ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is less then 10ng/ml, corresponding to a specific activity of 10Units/mg. | | Dimmers and Aggregates | 1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of FGF-acidic as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~17.5kD | | Purity | ~95% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Fibroblast Growth Factor 1 (Acidic) |
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Download Specifications for Fibroblast Growth Factor, Acidic, Recombinant, Human (Acidic Fibroblast Growth Factor, aFGF, FGF-alpha, FGFA, Beta-endothelial Cell Growth Factor, ECGF-beta, ECGFB, ECGF, ECGFA, FGF1, GLIO703, Heparin-binding Growth Factor 1, HBGF1, HBGF-1) |
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