Anti -Fibroblast Growth Factor, Basic (FGF Basic, FGFb, BFGF, Fibroblast Growth Factor 2,
FGF2, Heparin-binding Growth Factor 2, HBGF-2, Prostatropin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| FGF basic, otherwise known as FGF 2 (fibroblast growth factor 2) is a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kD, expressed by the majority of cells and tissues. FGF 2 is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumor vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis. | | | Catalog # | F4210-15H | | Applications | Suitable for use in Immunohistochemistry, ELISA, Functional Assays and Western Blot. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (Paraffin): 0.25-0.5ug/ml. Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer is recommened for this purpose. | | ELISA (Indirect or Sandwich): 0.5-2ug/ml | | Western Blot: 0.1-0.2ug/ml | | Functional Assays: 0.25-0.4ug/ml | | Optimal dilutions to be determined by the researcher. | | Recommended Pair | Capture: F4210-15H Fibroblast Growth Factor, Basic | | Detection: F4210-15H1 Fibroblast Growth Factor, Basic (Biotin) | | Positive Control | Human breast invasive ductal carcinoma | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O. | | Purity | Purified by affinity chromatography. | | Immunogen | Recombinant human FGF basic | | Specificity | Recognizes human FGF basic. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Basic Fibroblast Growth Factor |
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