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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -Fibroblast Growth Factor, Basic (FGF Basic, FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2, Prostatropin)

Anti -Fibroblast Growth Factor, Basic (FGF Basic, FGFb, BFGF, Fibroblast Growth Factor 2,
FGF2, Heparin-binding Growth Factor 2, HBGF-2, Prostatropin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
FGF basic, otherwise known as FGF 2 (fibroblast growth factor 2) is a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kD, expressed by the majority of cells and tissues. FGF 2 is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumor vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis.
Catalog #F4210-15H
ApplicationsSuitable for use in Immunohistochemistry, ELISA, Functional Assays and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Paraffin): 0.25-0.5ug/ml. Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer is recommened for this purpose.
ELISA (Indirect or Sandwich): 0.5-2ug/ml
Western Blot: 0.1-0.2ug/ml
Functional Assays: 0.25-0.4ug/ml
Optimal dilutions to be determined by the researcher.
Recommended PairCapture: F4210-15H Fibroblast Growth Factor, Basic
Detection: F4210-15H1 Fibroblast Growth Factor, Basic (Biotin)
Positive ControlHuman breast invasive ductal carcinoma
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
PurityPurified by affinity chromatography.
ImmunogenRecombinant human FGF basic
SpecificityRecognizes human FGF basic.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesBasic Fibroblast Growth Factor


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