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You are here:Home » Growth Factors, Cytokines » Growth Factors-FGF » Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding growth factor 2 precursor, Prostatropin)

Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2,
Heparin-binding growth factor 2 precursor, Prostatropin)

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Specifications

Fibroblast Growth Factor-basic (FGF-b or FGF-2) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo. Human FGF-b is a 17.2kD protein containing 154 amino acid residues.
Catalog #F4210-20
Amino acid sequence The first five N-terminal aa: Met-Ala-Ala-Gly-Ser. This corresponds to native human FGF-b: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERGVVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTGPGQKAILFLP MSAKS
Biological Activity Human FGF-b is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is  0.5ng/ml, corresponding to a specific activity of  2x10e6u/mg.
Contaminants Dimers and aggregates. 2%
Storage and Stability Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight17.2kD
SourceRecombinant Human (E. coli)
Purity98% by SDS-PAGE and HPLC analyses. Contaminants
Concentration~1mg/ml
FormSupplied as a liqlyophilized powder from 5mM TBS, pH 7.5. Reconstitution: Reconstitute with sterile, ddH2O, 0.1% HSA or BSA to .01mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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