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You are here:Home » Growth Factors, Cytokines » Growth Factors-FGF » Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding growth factor 2 precursor, Prostatropin)

Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2,
Heparin-binding growth factor 2 precursor, Prostatropin)

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Specifications

Catalog #F4210-20A2
Biological ActivityED50 range = 0.1-1.0ng/ml (Specific Activity: 1.0x10e7-1.0x10e6 units/mg) determined by the dose dependent proliferation of BALB/3T3 cells. Optimal concentration for individual application should be determined by a dose response assay.
SterilityFiltered prior to lyophilization through a 0.22 micron sterile filter.
Amino Acid SequencePALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH IKLQLQAEER GVVSIKGVCA YLAMKNREDG RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR GPGQKAILFL PMSAKS
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Further dilutions should be made in medium or buffered solution containing carrier protein such as PBS with 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17kD
SourceE. coli
Purity~95% by SDS-PAGE. Purified by sequential chromatography. Endotoxin: ~0.1ng/ug
ConcentrationNot determined
FormSupplied as a lyophilized powder, carrier-free. Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Further dilutions should be made in medium or buffered solution containing carrier protein such as PBS with 0.1% BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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