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Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding growth factor 2 precursor, Prostatropin)
Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding growth factor 2 precursor, Prostatropin)
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| Fibroblast Growth Factor-basic (FGF-b or FGF-2) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo. | | | Catalog # | F4210-20A4 | | Source | Recombinant human soluble basic FGF, aa134-288, expressed in E. coli. | | Molecular Weight | 17.3kD | | Biological Activity | Measured by its ability to stimulate proliferation of NIH3T3 cells. The ED50 of this effect is between 0.1-1ng/ml. | | Endotoxin | 1EU/ug (LAL) | | Applications | Suitable for use in ELISA. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 17.3kD | | Source | E. coli | | Purity | 98% (SDS-PAGE and HPLC) | | Concentration | As reported | | Form | Supplied as a lyophilized powder. Reconstitute with ddH2O or PBS. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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