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Fibroblast Growth Factor 2, Basic, Recombinant, Human (FGF2b)
Fibroblast Growth Factor 2, Basic, Recombinant, Human (FGF2b)
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| FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide | | | variety of cells including mesenchymal, neuroectodermal and endothelial cells. | | | Catalog # | F4210-24 | | Biological Activity | Cultures of fetal bovine heart endothelial (FBHE) cells grown for ve days in RD medium containing 2% FBS, 0.1ug/ml heparin, and 2ng/ml of this lot of FGF-2 showed > 12-fold more cells than cultures of FBHE cells grown in same medium without FGF-2. Results may vary depending on cell line used. Cell growth was assessed using an ATP luminescence assay | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. | | Molecular Weight | 17.5kD | | Source | Recombinant DNA expressed in yeast (S. cerevisiae). | | | Purity | Purified by heparin-sepharose chromatography. 95% as determined by HPLC and SDS-PAGE. | | | Concentration | ~0.5mg/ml | | | Form | Supplied as a liquid in 0.01mM Tris, pH 7.0, containing 1mM monothioglycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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