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You are here:Home » Growth Factors, Cytokines » Growth Factors-FGF » Fibroblast Growth Factor, Basic, Recombinant, Human (Basic Fibroblast Growth Factor, bFGF, FGFB, FGF2, Heparin-binding Growth Factor 2, HBGF-2)

Fibroblast Growth Factor, Basic, Recombinant, Human (Basic Fibroblast Growth Factor, bFGF,
FGFB, FGF2, Heparin-binding Growth Factor 2, HBGF-2)

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Specifications

Catalog #F4210-24C
SourceRecombinant corresponding to 155aa of human Fibroblast Growth Factor-basic expressed in E.coli s a single, non-glycosylated, polypeptide chain.
Molecular Weight~17.4kD
Biological ActivityThe ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake).
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1%(1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein 2. Analysis by RP-HPLC, using a standard solution of FGF-2 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.4kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesFibroblast Growth Factor 2 (Basic)


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