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Fibroblast Growth Factor, Basic, Recombinant, Human (Basic Fibroblast Growth Factor, bFGF, FGFB, FGF2, Heparin-binding Growth Factor 2, HBGF-2)
Fibroblast Growth Factor, Basic, Recombinant, Human (Basic Fibroblast Growth Factor, bFGF,
FGFB, FGF2, Heparin-binding Growth Factor 2, HBGF-2)
Pricing
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| Catalog # | F4210-24C | | Source | Recombinant corresponding to 155aa of human Fibroblast Growth Factor-basic expressed in E.coli s a single, non-glycosylated, polypeptide chain. | | Molecular Weight | ~17.4kD | | Biological Activity | The ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake). | | Dimmers and Aggregates | 1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1%(1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein 2. Analysis by RP-HPLC, using a standard solution of FGF-2 as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~17.4kD | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Fibroblast Growth Factor 2 (Basic) |
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