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Fibroblast Growth Factor 9, Recombinant, Mouse (FGF9)
Fibroblast Growth Factor 9, Recombinant, Mouse (FGF9)
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| Fibroblast Growth Factor-9 (FGF-9, Glial Activating Factor) is a heparin binding growth factor that stimulates the proliferation and activation of glial cells and other cells that express FGF receptors. Murine FGF-9 is a 23.3kD protein containing 205 aa residues. | | | Catalog # | F4211-75A | | Biological Activity | USBio's murine FGF-9 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is 0.5ng/ml, Corresponding to a specific activity of 2 x 10e6u/mg. | | Concentration Range for most in vitro applications | 0.1-1ng/ml | | Responding Cells (partial list) | Murine FGF-9 exerts full activity on both murine and human cells (i.e. Glial cells, 3T3 fibroblasts). | | AA Sequence | PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS | | Storage and Stability | Lyophilized powder may be stored at RT or desiccated at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40mM Tris, pH 8.0, aliquot and store at -20°C. Reconstituted product is stable for 12 months at | | -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 23.3kD | | Source | E. coli | | | Purity | 95% by SDS-PAGE and HPLC analyses. | | | Endotoxin | 0.1ng/ug. | | | Form | Supplied as a lyophilized powder from 20mM Tris, pH 8.0, 75mM sodium chloride. | | | Concentration | 1mg/ml | | | Reconstitution | To obtain a 2 mg/ml solution, add 0.5 µl of water per µg of protein. Dilution into solutions of lower ionic strength should be done immediately before use. For most in vitro applications, FGF-9 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml. |
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