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You are here:Home » Molecular Biology » Growth Factors-FGF » Fibroblast Growth Factor 9, Recombinant, Mouse (FGF9)

Fibroblast Growth Factor 9, Recombinant, Mouse (FGF9)

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Specifications

Fibroblast Growth Factor-9 (FGF-9, Glial Activating Factor) is a heparin binding growth factor that stimulates the proliferation and activation of glial cells and other cells that express FGF receptors. Murine FGF-9 is a 23.3kD protein containing 205 aa residues.
Catalog #F4211-75A
Biological ActivityUSBio's murine FGF-9 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is  0.5ng/ml, Corresponding to a specific activity of 2 x 10e6u/mg.
Concentration Range for most in vitro applications 0.1-1ng/ml
Responding Cells (partial list) Murine FGF-9 exerts full activity on both murine and human cells (i.e. Glial cells, 3T3 fibroblasts).
AA SequencePLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS
Storage and Stability Lyophilized powder may be stored at RT or desiccated at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40mM Tris, pH 8.0, aliquot and store at -20°C. Reconstituted product is stable for 12 months at
-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight23.3kD
SourceE. coli
Purity95% by SDS-PAGE and HPLC analyses.
Endotoxin0.1ng/ug.
FormSupplied as a lyophilized powder from 20mM Tris, pH 8.0, 75mM sodium chloride.
Concentration1mg/ml
ReconstitutionTo obtain a 2 mg/ml solution, add 0.5 µl of water per µg of protein. Dilution into solutions of lower ionic strength should be done immediately before use. For most in vitro applications, FGF-9 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml.


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