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You are here:Home » Molecular Biology » Growth Factors-FGF » Fibroblast Growth Factor 12, Recombinant, Human (FGF12, Fibroblast Growth Factor Homologous Factor 1, FHF1, Myocyte Activating Factor, FGF12B)

Fibroblast Growth Factor 12, Recombinant, Human (FGF12, Fibroblast Growth Factor
Homologous Factor 1, FHF1, Myocyte Activating Factor, FGF12B)

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Specifications

FGF12 (Fibroblast growth factor 12) is a member of the FGF superfamily of molecules which currently stands at 22 members. The FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF12 binds to IB2 (islet brain-2), a cellular kinase scaffold, and voltage gated sodium channels and also plays an important role in intracellular signaling and ion exchange.
Catalog #F4212-15B
Recombinant human FGF12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
SourceRecombinant corresponding to aa1-181 of human FGF12 (His-tagged) expressed in E.coli.
AA SequenceMGSSHHHHHH SSGLVPRGSH MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK
ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP
IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 3ug/ml 15% SDS-PAGE,
Optimal dilutions to be determined by the researcher.
Storage and StabilityCan be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Molecular Weight~22.6kD
SourceE. coli
PurityPurified 90% by SDS-PAGE.
Concentration~1mg/ml
FormSupplied as a liquid in 20mM tris pH 7.5, 10% glycerol, 1mM DTT, 2mM EDTA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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