Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Growth Factors, Cytokines » Growth Factors-FGF » Fibroblast growth factor 21, Recombinant, Human (FGF-21)

Fibroblast growth factor 21, Recombinant, Human (FGF-21)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Human FGF-21 Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a b-trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The c-DNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 shows limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the C-terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and b-hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).
A DNA sequence encoding the mature human FGF-21 (His 29-Ser 209; Accession # Q9NSA1) was fused with 5X histidines at the N-terminus. The protein was expressed in E. coli.
Catalog #F4212-93A
Molecular Mass Based on N-terminal amino acid sequencing, the recombinant human FGF-21 starts at the histidine tag and has a predicted molecular mass of approximately 20.2kD.
Endotoxin Level 1 EU/ug of the protein as determined by the LAL method.
Biological ActivityMeasured by its ability to stimulate the proliferation of NIH3T3 cells. The ED 50 for this effect is typically 0.12-0.6ug/ml in the presence of 5ug/ml of rmKlotho-beta
Measured in a cell proliferation assay using human FGF RIIIc transfected BaF3 mouse pro­B cells. The ED50 for this effect is typically 0.06 ­ 0.4ug/ml in the presence of rmKlotho-beta
Storage and Stability:
Lyophilized powder stored at -20°C to -70°C is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Product reconstituted under sterile conditions is stable for 1 month at 4°C and for 3 months at --20°C to -70°C. Avoid freeze-thaw cycles.
SourceE. coli.
Purity> 97%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0 EU per 1ug of the protein as determined by the LAL method.
FormSupplied as a lyophilized powder in 10 mM MES, 50 mM sodium sulfate, 0.2 mM EDTA and
0.2 mM DTT, pH 6.5. Reconstitute with PBS to a concentration of 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links