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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Fibroblasts

Anti -Fibroblasts

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC FC IF
Catalog #F4213
ApplicationsSuitable for use in Immunofluorescence, FACS, and the assessment of thymic epithelial culture contamination with fibroblasts and the identification of cells of mesodermal origin in the fetus. Other applications have not been tested.
Recommended DilutionsImmunofluorescence (Acetone fixed tissue): 1:100-1:200
FACS: 1:100 using fresh or acetone fixed tissue; do not use trypsin to prepare adherent cell lines, use a non-enzymatic detactment procedure.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1507
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide
PurityPurified by ion exchange chromatography.
ImmunogenWhole human thymic stoma cells.
SpecificityRecognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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