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You are here:Home » Antibodies » Abs to Cell Markers » Anti -FIBROBLASTS, EPITHELIAL CELLS (PE)

Anti -FIBROBLASTS, EPITHELIAL CELLS (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leukocytes. F4213-01 has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation
giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes.
Catalog #F4213-01
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat, 1:10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9A170
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH7.4 1% BSA, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman foreskin fibroblasts.
SpecificityRecognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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