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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH FC

In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leukocytes. F4213-30B has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation
giving a positive reaction with dedifferentiated human chondrocytes and negative with differentiated chondrocytes.
Catalog #	F4213-30B
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul
	Immunohistochemistry (Frozen): 1:100
	Optimal dilutions to be determined by the researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	9A170
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Human foreskin fibroblasts
Specificity	Recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. Does not recognize mouse or rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.