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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Fibroblasts, Epithelial Cells

Anti -Fibroblasts, Epithelial Cells

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leukocytes. F4213-30B has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation
giving a positive reaction with dedifferentiated human chondrocytes and negative with differentiated chondrocytes.
Catalog #F4213-30B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (Frozen): 1:100
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9A170
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman foreskin fibroblasts
SpecificityRecognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. Does not recognize mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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