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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IP IH

Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells.
Catalog #	F4213-60
Applications	Suitable for use Western Blot, Immunohistochemistry ELISA and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:500
	Immunohistochemistry: Frozen and paraffin sections
	Immunoprecipitation: 1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	8k221
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in PBS., pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Ascites
Immunogen	Fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts.
Specificity	Recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. Does not react with vascular smooth muscle cells. May be used for removal of fibroblasts from human cultured cells by inhibition of fibroblast adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.