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You are here:Home » Antibodies » Antibodies-Cell Markers » Anti -Fibroblast Surface Protein

Anti -Fibroblast Surface Protein


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Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IH
Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells.
Catalog #F4213-60
ApplicationsSuitable for use Western Blot, Immunohistochemistry ELISA and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Immunohistochemistry: Frozen and paraffin sections
Immunoprecipitation: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No8k221
ConcentrationNot Determined
FormSupplied as a liquid in PBS., pH 7.4, 1% BSA, 0.1% sodium azide.
ImmunogenFusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts.
SpecificityRecognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. Does not react with vascular smooth muscle cells. May be used for removal of fibroblasts from human cultured cells by inhibition of fibroblast adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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