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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites IH

Prolyl 4-hydroxylase (P4-H), an enzyme localized to the endoplasmi reticulum (ER), catalyses the co- and post-translational hydroxylation of proline residues in Xaa-Pro-Gly sequences of collagens and proteins with collagen-like aa sequences. Vertebrate P4-H consists of two distinct polypeptides; the catalytically more important, glycosylated alpha-subunit (64kD) and the beta-subunit (60kD) which belongs to the multifunctional enzyme protein disulphide isomerase (PDI). The beta subunit is a major cellular thyroid hormone-binding protein and shows partial homology with a phosphoinositide-specific phospholipase C, thioredoxins, and the estrogen-binding domain of the estrogen receptor.It catalyzes the formation, breakage and rearrangement of disulfide bonds. It also acts as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner at high concentrations. Other function of the protein includes its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. The COOH-terminus of the beta subunit has the aa sequence Lys-Asp-Glu-Leu, which was recently suggested to be necessary for the retention of a polypeptide within the lumen of the endoplasmic reticulum.
Catalog #	F4213-60A
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
	Optimal dilutions to be determined by the researcher.
Positive Control	Thymus
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	11E194
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid, 0.05% sodium azide.
Purity	Ascites
Immunogen	Synthetic peptide corresponding to human prolyl 4-hydroxylase. Cellular Localization: Membrane.
Specificity	Recognizes proteins of 43kD and 72-80kD.Recognizes a surface protein on fibroblasts (synovial, foreskin and thymic), fibroblast cell lines, tissue macrophages, and 95% of peripheral blood monocytes. Does not stain lymphocytes or human epithelial cells but does stain malignant fibrosarcoma tissue.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.