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You are here:Home » Antibodies » Antibodies-Fibronectin » Anti -Fibronectin, Heparin Binding Domain 2

Anti -Fibronectin, Heparin Binding Domain 2


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Fibronectin is an adhesive glycoprotein with a molecular mass of 440kD. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).
Catalog #F4215-01N
ApplicationsSuitable for use in ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 1:4000 (1,2,3,4)
Western Blot: 1:100 (1)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone NoA32
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenBovine corneal endothelial cells.
SpecificityHighly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). Cross-reacts with human fibronectin. Other species have not been tested. The epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of clone 6F104.
Can be used in ELISA, Western blotting, immunoprecipitation and immunostaining of frozen PLP-fixed sections of bovine and human tissues. The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with clone 6F104.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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