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You are here:Home » Antibodies » Abs to Fibronectin » Anti -Fibronectin

Anti -Fibronectin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression.
Catalog #F4215-04B
ApplicationsSuitable for use in Immunoblot and Immunohistochemistry (frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment)
Recommended DilutionImmunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature.
Positive ControlHuman skin, ulcerating tissue.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F124
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PurityCulture supernatant
ImmunogenCultivated human fibroblasts.
SpecificityAntigen recognized human in Species (tested so far). Reacts with the cell binding part of the fibronectin molecule. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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