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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH

Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression.
Catalog #	F4215-04B
Applications	Suitable for use in Immunoblot and Immunohistochemistry (frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment)
Recommended Dilution	Immunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature.
Positive Control	Human skin, ulcerating tissue.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F124
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Culture supernatant
Immunogen	Cultivated human fibroblasts.
Specificity	Antigen recognized human in Species (tested so far). Reacts with the cell binding part of the fibronectin molecule. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.