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You are here:Home » Antibodies » Abs to Fibronectin » Anti -Fibronectin

Anti -Fibronectin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IF
Fibronectin is a dimeric glycoprotein which is present in cells, extracellular matrix, and blood. It possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing.
Catalog #F4215-05
ApplicationsSuitable for use as in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-2ug/ml for 2 hours at RT.
Immunoprecipitation (native and denatured): 2ug/mg protein lysate. Use Protein G.
Optimal dilutions to be determined by the researcher.
Positive Control
SW156 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.295
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See F4215-05X.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman fibronectin purified from serum by affinity chromatography on gelatin-sepharose. Cellular Localization
SpecificityRecognizes both the plasma and cellular forms of human fibronectin. Blocks the fibronectin-mediated cell attachment by interfering with the attachment of fibronectin to its receptor on the cell surface: not by disrupting the collagen-fibrinogen interaction.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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