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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IF

Fibronectin is a dimeric glycoprotein which is present in cells, extracellular matrix, and blood. It possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing.
Catalog #	F4215-05
Applications	Suitable for use as in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions	Western Blot: 1-2ug/ml for 2 hours at RT.
	Immunoprecipitation (native and denatured): 2ug/mg protein lysate. Use Protein G.
	Optimal dilutions to be determined by the researcher.
Positive Control
	SW156 cells
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.295
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See F4215-05X.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose. Cellular Localization
Specificity	Recognizes both the plasma and cellular forms of human fibronectin. Blocks the fibronectin-mediated cell attachment by interfering with the attachment of fibronectin to its receptor on the cell surface: not by disrupting the collagen-fibrinogen interaction.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.