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You are here:Home » Antibodies » Abs to Fibronectin » Anti -Fibronectin

Anti -Fibronectin

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Fibronectin is a dimeric glycoprotein (~440kD) which is present in cells, extracellular matrix, basement membranes, interstitial connective tissue matrix and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Catalog #F4215-10A
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot (Reduced and non-reduced): 0.5-1ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F179
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See F4215-10AX.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant fibronectin protein. Cellular Localization
SpecificityRecognizes human fibronectin. Species Crosseactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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