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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

Fibronectin is a dimeric glycoprotein (~440kD) which is present in cells, extracellular matrix, basement membranes, interstitial connective tissue matrix and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Catalog #	F4215-10A
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot (Reduced and non-reduced): 0.5-1ug/ml for 2hrs at RT.
	Immunohistochemistry (Formalin/paraffin): 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3F179
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See F4215-10AX.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant fibronectin protein. Cellular Localization
Specificity	Recognizes human fibronectin. Species Crosseactivity: mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.