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You are here:Home » Antibodies » Abs to Fibronectin » Anti -Fibronectin (BSA & Azide Free)

Anti -Fibronectin (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Fibronectin is a dimeric glycoprotein (~440kD) which is present in cells, extracellular matrix and blood (1). It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing.
Catalog #F4215-10AX
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlNormal kidney
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F179
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No stabiilizing proteins and no preservatives added. Also available with BSA and azide. See F4215-10A.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant fibronectin protein. MW
SpecificityRecognizes fibronectin. Species Crosseactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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