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You are here:Home » Antibodies » Antibodies-Fibronectin » Anti -Fibronectin (FN, Cold-insoluble Globulin, CIG, FN1) (APC)

Anti -Fibronectin (FN, Cold-insoluble Globulin, CIG, FN1) (APC)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state, but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.
Catalog #F4215-13B
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 10ul labels up to 1x10e6 cells. For intracellular staining, cells must first be fixed with 4% paraformaldehyde buffer and permeabilized with 0.1% saponin saline buffer.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Harvest cells and wash twice in saline buffer.
2. Cell surface staining may be done at this point following the manufacturer’s staining procedure.
3. Resuspend up to 1x10e6 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins.
4. Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer.
5. After permeabilization, add 10ul of F4215-13B and incubate the cells for 30 minutes at RT in the dark.
6. Wash the cells twice with 0.1% saponin saline buffer.
7. Resuspend the cells in saline buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.
Clone TypeMonoclonal
Clone No11C16
ConcentrationNot determined
FormSupplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified, human plasma Fibronectin.
SpecificityRecognizes human Fibronectin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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