| Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state, but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression. |
|
| Catalog # | F4215-13B |
| Applications | Suitable for use in Flow Cytometry. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 10ul labels up to 1x10e6 cells. For intracellular staining, cells must first be fixed with 4% paraformaldehyde buffer and permeabilized with 0.1% saponin saline buffer. |
| Optimal dilutions to be determined by the researcher. |
| Flow Cytometry Protocol | 1. Harvest cells and wash twice in saline buffer. |
| 2. Cell surface staining may be done at this point following the manufacturer’s staining procedure. |
| 3. Resuspend up to 1x10e6 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins. |
| 4. Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer. |
| 5. After permeabilization, add 10ul of F4215-13B and incubate the cells for 30 minutes at RT in the dark. |
| 6. Wash the cells twice with 0.1% saponin saline buffer. |
| 7. Resuspend the cells in saline buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody. |
| Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 11C16 |
| Host | Mouse |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC). |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Purified, human plasma Fibronectin. |
| Specificity | Recognizes human Fibronectin. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
