Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry, Inactivation or inhibition studies in cell culture. Other applications not tested.
Recommended Dilution
Immunocytochemistry: 5-20ug/ml Immunohistochemistry: 5-20ug/ml. Not tested on paraffin sections. Western Blot: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human-fibronectin from amnion liquid.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes human fibronectin and with a peptic fragment 11.5kD from human fibronectin comprising the cell attachment site. It inhibits the attachment of normal rat kidney cells to human fibronectin coated plastic surface. Binding of the antibody to fibronectin cannot be inhibited by cell adhesion peptides from fibronectin having the sequence Arg-Gly-Asp of Gly-Arg-Gly-Asp-Ser. Species Reactivity: Human
References
1. Engvall, E. et al. (1977) Int. Cancer J. 20, 1–5. 2. Pierschbacker, M. D. et al. (1981) Cell 26, 259–267. 3. Pierschbacker, M. D. et al. (1982) J. Biol. Chem, 257, 9593–9597. 4. Pierschbacker, M. D. et al. (1983) Proc. Natl. Acad. Sci. USA, 80, 1224- 1227. 5. Pierschbacker, M. D. et al. (1984) Nature 309, 30–33. 6. Hayman, E. G. et al. (1985) J. Cell Bio. 100, 1948–1954. 7. Ruoslahti, E. et al. (1986) Cell 44, 517–518. 8. Hynes, R. (1985) Ann. Rev. Cell Biol. 1, 67–90.
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