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You are here:Home » Antibodies » Abs to Fibronectin » Anti -Fibronectin

Anti -Fibronectin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF RIA
Rabbit polyclonal antibodies against Fibronectin extracted and purified from bovine plasma.
Catalog #F4215-44
ApplicationsSuitable for use in Immunohistochemistry, Immunofluorescence, Western Blot, RIA and ELISA. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1:80 dilution for immunofluorescent staining of frozen bovine skin and liver tissues. Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 min) acetone or methyl-carnoy fixation, traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only.
Western Blot: 1:1000 of 2% deoxycholate and 10% SDS, 6M urea extraced bovine cell cultures. Detects bands at ~220kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2 No preservatives.
PurityPurified by Protein G chromatography.
ImmunogenFibronectin extracted and purified from bovine plasma.
SpecificityRecognizes bovine Fibronectin. Shows <0.1% reactivity with bovine Collagen Types I and III by radioimmunoassay.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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