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You are here:Home » Antibodies » Abs to Fibronectin » Anti -Fibronectin

Anti -Fibronectin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Fibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
Catalog #F4215-47
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.05-1ug/ml. Detected Fibronectin in 15ul of normal rat serum.
Optimal dilution determined by the researcher.
Recommended Secondary AntibodiesI1904-46E IgG, H&L (HRP) Pab DkxRb
I1904-46K IgG, H&L, X-Adsorbed (HRP) Pab DkxRb
Recommended Positive ControlNormal rat serum
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified rat plasma fibronectin.
SpecificityRecognizes rat fibronectin at ~262kD. No crossreactivity with other rat plasma proteins as verified via Western Blot analysis. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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