| Fibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. |
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| Catalog # | F4215-47 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.05-1ug/ml. Detected Fibronectin in 15ul of normal rat serum. |
| Optimal dilution determined by the researcher. |
| Recommended Secondary Antibodies | I1904-46E IgG, H&L (HRP) Pab DkxRb |
| I1904-46K IgG, H&L, X-Adsorbed (HRP) Pab DkxRb |
| Recommended Positive Control | Normal rat serum |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Purified rat plasma fibronectin. |
| Specificity | Recognizes rat fibronectin at ~262kD. No crossreactivity with other rat plasma proteins as verified via Western Blot analysis. Species Crossreactivity: mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
