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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E IF

Fibronectin is a protein which can bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin is present in the body in two forms; plasma
fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Interaction with Tenascin-R mediates inhibition of cell adhesion and neurite outgrowth.
Catalog #	F4215-48
Applications	Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:50-1:500
	Immunofluorescence: 1:5-1:20
	Optimal dilutions to be determined by the researcher.
Storage and Stability
	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, before the addition of glycerol to 40%. Labeled with FITC.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Fibronectin
Specificity	Recognizes human fibronectin. Species Crossreactivity: canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.