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You are here:Home » Antibodies » Abs to Fibronectin » Anti -Fibronectin (FN, Cold-insoluble Globulin) (Biotin)

Anti -Fibronectin (FN, Cold-insoluble Globulin) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit E B IP IH IF
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Catalog #F4215-54J
Cellular Localization Extracellular matrix.
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
Form0.01M Na phosphate, 0.25M NaCl, pH7.6, 15mg/ml BSA. Label with Biotin.
PurityWhole antisera
ImmunogenFibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.
SpecificityNegligible crossreactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with most Mammals due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, Fibronectin 1, FINC, FN 1, FN1, LETS, MSF, Transformation Sensitive Protein


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