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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IP IH IF RIA

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kD. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
Catalog #	F4215-90
Applications	Suitable for use in Western Blot, Immunoprecipitation, RIA
	Indirect immunofluorescence, Immunohistochemistry and Immunoperoxidase.
	Other applications have not been tested.
Recommended Dilutions
	Immunohistochemistry: For best results use cryostat sections fixed with either acetone or 4% formaldehyde.
	Has not been tested on paraffin embedded tissue.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	2Q610
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid.
Purity	Purified
Immunogen	Proteins secreted by cultured human embryonic fibroblasts
Specificity	Recognizes whole human fibronectin and many of its proteolytic fragments. Specifically recognizes N-terminal fibrin-and heparin-binding 29kD domain. Specificity established by Western blotting with purified domain from two different sources.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.