Anti -FBLN5 (Fibulin-5, FIBL-5, Developmental Arteries and Neural Crest EGF-like Protein,
Dance, Urine p50 Protein, UP50, DANCE, UNQ184/PRO210)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| Fibulin 5, also known as DANCE and EVEC, is a secreted 55kD matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis. Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44aa spacer region, five more tandem EGF-like domains, and a 115aa Fibulin-like C-terminal region. Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5. Fibulin 5 i s expressed by smooth muscle cells and endothelial cells of the developing asculature as well as by migrating neural crest cells and l unginterstitial fibroblasts. It is downregulated in the adult vasculature but is re-expressed at aortic branching points, in the uterus, and at sites of mechanical or at herosclerotic injury. The RGD motif of Fibulin 5 binds to several cell surface Integrins including aVb3, aVb5, a9b1, a4b1, and a5b1. The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix. Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2, and the crosslinking lysyl oxidase-like enzymes LOXL1, 2, and 4 along Fibrillin microfibrils. In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevent s Fibulin 5 from interacting with Fibrillin-1 microfibrils. Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation a migration and by limiting vascular sprouting. Fibulin 5 is downregulated in many malignancies. When experimentally re-expressed, however, Fibulin 5 enhances tumor cell invasiveness and cooperates with TGF-b i n initiating and promoting epithelial-mesenchymal transition (EMT). Defects in Fibulin 5 expression or function can result in a loss of connective tissue Integrity, cardiac elasticity, and ability to remodel the vasculature after injury. | | | Catalog # | F4350-35C | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:32,000 | | Western Blot: 0.1-0.3ug/ml, observed in human colon, heart and ovary lysates on ~50kD bands | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-RPIKGPREIQLDLE, from human FBLN5, at the internal region (near the C-terminal) of the protein (NP_006320.2). | | Specificity | Recognizes human FBLN5. Species sequence homology: Canine, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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